HPLC-DAD assay of phenols profile in Antennaria dioica (L.) Gaertn

Antennaria dioica ( Asteraceae family) – is a perennial herb, commonly found in dry grasslands and sandy or stony places from Eurasian areas. It known traditional medicine as antioxidant, diuretic, choleretic anti-inflammatory remedy. This species should be reconsidered possible sources of phenols, mainly flavonoids hydroxycinnamic acids. Thus, the aim this study was to validate chromatographic method for detection phenols their identification A. herb. HPLC-DAD evaluated terms linearity, precision, repeatability, accuracy, LOD LOQ. The calibration curves thirteen reference substances were linear (R 2 > 0.99) over range 5–400 µg/mL, LODs LOQs 0.1–0.3 µg/mL 0.2–1.0 respectively. During assay two flavones luteolin, apigenin; flavonol quercetin three its glycosides rutin, hyperoside isoquercitrin; coumarins: coumarin umbelliferone; five acids chlorogenic, caffeic, p -coumaric, trans -ferulic rosmarinic identified phytochemical confirms that plant material rich source phenolic compounds.